Flow-cell loading converter
Convert a loading concentration from one platform to another by proportion. You supply each platform’s recommended optimum — no instrument values are baked in.
How it works
Formula
target loading = source loading × (target optimum ÷ source optimum). If you load at your source platform’s optimum, you get the target platform’s optimum; otherwise the same proportional offset is preserved.
Worked example
Loading at 1500 pM where the source optimum is 1500 pM and the target optimum is 750 pM: 1500 × (750 ÷ 1500) = 750 pM.
When to use it
When moving a library between sequencers and you want to keep the same relative loading, scaled to each platform’s recommended concentration.
Sensible defaults
The default optima are placeholder example values only — they are NOT authoritative instrument specifications. Enter the recommended loading concentrations from your own current platform protocols.
Source
Conversion is proportional only. No per-instrument concentrations are embedded; all recommended optima are user-supplied — always confirm against your current protocol.
FAQ
- Why doesn’t the tool know each platform’s recommended concentration?
- Because those values change with chemistry versions and protocols and would go stale. You supply the current recommended optima from your own documentation, and the tool just does the proportional conversion.
- What units should I use?
- Any consistent unit (pM is typical). The result comes out in the same unit you enter, since it is a ratio-based conversion.