Where do I find the nmol amount?

On the oligo synthesis spec sheet from the vendor — it lists the actual nmol (or OD/µg) delivered, which varies per synthesis.

What concentration should I resuspend to?

A 100 µM stock is common; you then dilute to a 10 µM working stock for most PCR. Pick whatever your protocol expects.

Oligo resuspension calculator

Work out the volume of buffer to add to a dried oligo to reach a target stock concentration, from the amount supplied in nmol.

How it works

Formula

volume = amount ÷ concentration. With amount in nmol and concentration in µM, volume (µL) = (nmol ÷ µM) × 1000. A handy shortcut: for a 100 µM stock, µL = nmol × 10.

Worked example

A 10 nmol oligo to 100 µM: (10 ÷ 100) × 1000 = 100 µL. Checking: 10 nmol in 100 µL = 100 µmol/L = 100 µM.

When to use it

When resuspending a freshly synthesised, lyophilised oligo to a known stock concentration before making working dilutions for PCR or sequencing.

Sensible defaults

Defaults resuspend a typical 10 nmol synthesis to a 100 µM stock (→ 100 µL). Read the exact nmol delivered from your oligo spec sheet.

FAQ

Where do I find the nmol amount?: On the oligo synthesis spec sheet from the vendor — it lists the actual nmol (or OD/µg) delivered, which varies per synthesis.
What concentration should I resuspend to?: A 100 µM stock is common; you then dilute to a 10 µM working stock for most PCR. Pick whatever your protocol expects.