Reverse complement
Get the reverse complement of a DNA sequence (5′ → 3′), with its length and GC content. Accepts A, C, G, T and N.
How it works
Formula
Complement each base (A↔T, C↔G, N→N), then reverse the order so the result reads 5′ → 3′.
Worked example
ATCGGA → complement TAGCCT → reverse TCCGAT. So the reverse complement of ATCGGA is TCCGAT.
When to use it
When you need the opposite strand: designing a reverse primer, reading a feature annotated on the minus strand, or checking an oligo against its target.
Sensible defaults
The default ATCGGA returns TCCGAT — a short example you can verify by hand.
FAQ
- Is it case sensitive?
- No. Input is read case-insensitively and the result is returned in uppercase.
- What about ambiguous bases?
- N is supported and maps to N. Other IUPAC ambiguity codes are not accepted by this tool.